It might seem like a paradox that you want a dark field to see things with an expensive microscope. As [IMSAI Guy] explains, a dark field microscope doesn’t make the subject dark. It makes the area surrounding the subject dark. After selling his expensive microscope, he found he missed having the capability, so he decided to make one cheaply. You can see how he did it in the video, below.
Dark field microscopy gives better contrast and resolution by discarding light that shines directly through or reflects directly from a sample. The only light you see is any that scatters. If you think about a normal microscope, you can imagine a cone of light coming from the top or the bottom. The tip of the cone hits the sample and then spreads back out into another cone of light. What hits your eye –well, actually, the eyepiece — is all the light from that cone. In a dark field instrument, the illumination cone is hollow — the light is just a ring. That means any light the sample doesn’t scatter gets blocked by a stop in the objective. When there is no sample, there’s no unblocked light, so you see…
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